The Protective Effect of Rosewater against DNA Damages and Oxidant and Antioxidant Parameters of Rats Exposed to Chlorpyrifos Ethyl

Abstract

Objective: The aim of this study was to determine both the protective effect of rose water (RW) against DNA damage in the tissues of rats exposed to chlorpyrifos-ethyl (CPE) and RW’s effect on the oxidant and antioxidant levels in the blood serum and brain tissues of those same rats. Methods: In this experimental study, 32 mature male wistar albino rats were divided into 4 groups: group I, control; group II, CPE; group III, RW; and group IV, CPE+RW. The parameters of DNA tail intensity and DNA tail moment were analysed in blood samples by comet assay. Glutathione S-transferase (GST), catalase (CAT), and malondialdehyde (MDA) levels in brain tissues were examined. In blood serum, the levels of melatonin (MT) from 3-nitrotyrosine (3-NT) were determined. Results: In the CPE+RW group, the MDA and 3-NT levels in the brain tissues were significantly reduced (p<0.001), while the MT, GST, and CAT levels were significantly higher (p<0.001) compared to those of the CPE group. When the control and RW groups were compared, the CAT, GST, and MT levels were significantly higher (p<0.001) in the RW group, while the MDA and 3-NT levels were significantly lower (p<0.001). Conclusion: In rats, RW had positive effects on oxidant damage created by CPE. Both the DNA tail intensity and DNA tail moment in the CPE group were significantly higher (P<0.001) compared to those measures for the control group.